WG5 - Toxicity

WG5 - Toxicity of UCNPs

Defining the requirements and providing safety guidelines by assessing the nanotoxicity of UCNMs

The major objectives for the Toxicity of UCNPs working group are the following :

 

Evaluation of the UCNM interaction with cells

• Life-cell imaging (3–7 days) for the evaluation of UCNM’s uptake, cell division and cell death.

• Comparison with currently used commercial labels for long-term monitoring of cell such as quantum dots.

 

Cytotoxicity testing

• Evaluation of the cytotoxic and genotoxic potential of UCNMs on relevant cell types, both after short term (24h) and long term (4 weeks) exposure.

Safety Evaluation

• Biosafety of UCNMs will be evaluated by analysis of experimental cytotoxicity data. Available information will be integrated for an effective risk assessment.

 

Reference materials

• Well characterized highly photoluminescent efficient UCNMs will be used as standards for the biocompatibility evaluations.

 

The working group achievements :

 

WG5 Workshop-Training Workshop on Cytotoxicity, 19-22 October 2015, Graz, Austria.

 

STSM

  • Federico Herrera. “Microinjection of flatworms for testing cytotoxicity and behavior of UCNPs in vivo”. Ref. COST-STSM-CM1403-140216-071751.
  • Cynthia Kembuan. “Investigation of the Influence of a Silica Shell on the Cytotoxicity of Upconversion Nanoparticles”. Ref. COST-STSM-CM1403-30002
  • Anna Ekner-Grzyb. “A battery of toxicity tests for the assessment of the biocompatibility of the up-converting nanoparticles”. Ref. COST-STSM-CM1403-30006

 

Projects-National Funded project (FCT)

  • “Nanoheaters and nanothermometers playing together: towards applications in Brownian motion and hyperthermia” (2016-2019). PTDC/CTM-NAN/4647/2014. Luis Dias Carlos (project coordinator), Helena Oliveira (research team), Federico Herrera (research team)

 

Publications

  • ML Debasu, CDS Brites, S Balabhadra, H Oliveira, J Rocha and LD Carlos. Nanoplatforms for Plasmon-Induced Heating and Thermometry. Chemnanomat, In Press

 

Conferences

  • R Letra-Vilela, A C Almeida, J Branco-Santos, A M Rocha, F Herrera. The flatworm Schmidtea mediterranea as an in vivo model for the toxicity of upconverting nanoparticles. UPCON16, Wroclaw.
  • H. Oliveira. Unveiling the biocompatibility of upconverting nanomaterials: finding the best way forward. UPCON16, Wroclaw
  • Toxicological assessment of UCNPs in vitro, Fröhlich E., Meindl C., Leitinger G., Roblegg E., UPCON16, Wroclaw

 

WG5 LEADERS

 

Dr. Helena Oliveira

Department of Biology, CESAM (Centre for Environmental and Marine Studies) & CICECO (Aveiro Institute of Materials)

University of Aveiro

3810-193 Aveiro, Portugal

Phone : +351 234 247186

Email : holiveira@ua.pt

 

Dr. Federico Herrera

Cell Structure and Dynamics Laboratory

Instituto de Tecnologia Química e Biológica (ITQB-UNL)

Av. da República, Estação Agronómica Nacional

2781-901 Oeiras, Portugal

 

Phone: +(351) 21 446 9300

e-mail: fherrera@itqb.unl.pt

 

 

 

EUROPEAN UPCONVERSION NETWORK

COST Action CM1403 (2014-2018)

Chair: dr Hans Gorris (University of Regensburg, Germany)

Vice-Chair: prof.Tero Soukka (University of Turku, Finland)

COST Science Officer: Dr. Lucia Forzi (Bruseels, Belgium)

STSMs Manager: dr hab. Artur Bednarkiewicz (PAS & WCB EIT+, Poland)

CONTACT

info @ ucnp.eu

http://www.cost.eu/COST_Actions/cmst/CM1403

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