Eleonore Frohlich (AUSTRIA)

Eleonore Fröhlich (Austria)

PLACE OF WORK:

 

Center for Medical Research, Stiftingtalstr. 24, A-8010 Graz

 

 

CONTACT

 

Phone : +43 316 385735176

 

Email : Eleonore.froehlich (at) medunigraz.at

 

COST ROLE : MC Member (Austria), WG 5- Toxicity

Description of the Organisations and PI

 

The Center for Medical Research (ZMF) provides a first class infrastructure for patient-oriented research. The modern building which has been specially built for the demands of biomedical research at the centre of the University Campus has available more than 4,000 m2 of optimally equipped laboratory and office space as well as excellent core facilities for services. The center comprises well equipped laboratory and office space catering for cutting edge basic and applied biomedical research and clinical research. Five Core Facilities (Molecular biology, Imaging, Ultrastructure Analysis, Mass Spectrometry, and Computational Bioanalytics) supplying most of the date equipment and well trained permanent technical staff are available to all researchers. All Core Facilities are certified by ISO 9001:2008.

The Medical University of Graz (MUG) comprises 16 University Research Institutes and 20 Clinical Departments. The University is the employer of 1832 highly qualified persons, of which 741 make up the academic stuff. Approximately 4190 students are currently enrolled in the key study programs. Research activities cover a broad spectrum in clinical as well as pre-clinical fields. Furthermore, the university acts as a strong partner in the Central European region and coordinates a major research initiative involving all medical faculties Balkan countries. The MUG established specific institution in order to better support research activities, namely the Biobank Graz (Biobank), the Institute of Biomedical Research (BBF) and the Center for Medical Research (ZMF). The BBF enables researchers to conduct in vivo studies on rodents, rabbits and sheep of standardized high quality as well as to use a panel of alternative in-vitro assays. The Biobank has a collection of biological samples with associated data in a structured usable form. With some six million biological samples, it counts as one of the biggest establishments of its kind in Europe.

Eleonore Fröhlich holds a Diploma in Biochemistry from the Eberhard-Karls University, Tübingen (Germany) and a medical degree from the Karl-Ruprechts-University Heidelberg (Germany). Eleonore Fröhlich specialized in Histology, Embryology and Anatomy with research on retina function, on thyroid carcinoma and on melanoma. After being group leader for drug development in metabolic liver diseases at Oridis Biomed, Graz (Austria), she is, since 2007, Director of the Core Facility Imaging and extraordinary professor at the Institute of Anatomy of the University of Tübingen. Since 2013 she acts also as Scientific advisor of the Animal Welfare Body of the Medical University of Graz according to Directive 2010/63/EU on the protection of animals used for scientific purposes and as Key researcher at the Research Center Pharmaceutical Engineering, Graz. Eleonore Fröhlich is involved in national and international projects on nanotoxicology as principal investigator or scientific coordinator, and in the development of standardized testing protocols for nanoparticles as official data provider for the OECD.

 

Description and scientific profiles of the group

 

The group has expertise with a variety of light microscopical techniques (life cell imaging, cell monitoring, confocal microscopy), physiologically relevant culture models (particularly for inhalation), and toxicological assays (cytotoxicity, genotoxicity, hemocompatibility, immunotoxicity). Focus of the group is the biological assessment of nano- and microparticles. Biocompatibility testing is performed according to ISO 10993 guidelines.

Research infrastructure

 

The Core Facility Imaging at the Center of Medical Research is equipped with S1 and S2 cell culture labs (Laminar flow hoods, CO2 incubator, etc.), automated microscopes for cell monitoring, life cell imaging, various conventional epifluorescence and brightfield light microscopes, and the confocal laser scan microscopes LSM510 Meta and Nikon A1R. LSR II, Cytoflex, FACS Aria are used for flow cytometry and cell sorting. In addition to that, infrastructure for tissue embedding, sectioning, automated staining, image acquisition and image analysis is available. The unit has free access to the shared infrastructure at the Center for Medical Research such as ultracentrifuges, photometer, fluorescence and chemoluminescence readers, etc.

 

Most significant recently published papers:

  • Fröhlich, E. Value of phagocyte function screening for immunotoxicity of nanoparticles in vivo. INT J NANOMED. 2015; 10: 3761-3778.
  • Meindl, C; Kueznik, T; Bösch, M; Roblegg, E; Fröhlich, E. Intracellular calcium levels as screening tool for nanoparticle toxicity. J Appl Toxicol. 2015; 2015 Oct;35(10):1150-9.
  • Mrakovcic, M; Meindl, C; Leitinger, G; Roblegg, E; Fröhlich, E. Carboxylated Short Single-Walled Carbon Nanotubes But Not Plain and Multi-Walled Short Carbon Nanotubes Show in vitro Genotoxicity. Toxicol Sci. 2015; 144(1):114-127
  • Fröhlich, E; Meindl, C; Wagner, K; Leitinger, G; Roblegg, E. Use of whole genome expression analysis in the toxicity screening of nanoparticles. Toxicol Appl Pharmacol. 2014; 280(2):272-284
  • Prietl, B; Meindl, C; Roblegg, E; Pieber, TR; Lanzer, G; Fröhlich, E. Nano-sized and micro-sized polystyrene particles affect phagocyte function. Cell Biol Toxicol. 2014; 30(1):1-16
  • Fröhlich, E; Bonstingl, G; Höfler, A; Meindl, C; Leitinger, G; Pieber, TR; Roblegg, E, Comparison of two in vitro systems to assess cellular effects of nanoparticles-containing aerosols. Toxicol In Vitro. 2013; 27(1):409-417
  • Fröhlich, E; Meindl, C; Höfler, A; Leitinger, G; Roblegg, E. Combination of small size and carboxyl functionalisation causes cytotoxicity of short carbon nanotubes. Nanotoxicology. 2013; 7(7):1211-1224
  • Mrakovcic, M; Absenger, M; Riedl, R; Smole, C; Roblegg, E; Fröhlich, LF; Fröhlich, E. Assessment of long-term effects of nanoparticles in a microcarrier cell culture system. PLoS One. 2013; 8(2):e56791-e56791

Related research grants:

 

  • HORIZON2020 FET‐Open 2014-2015 RIA, CONQUER: Contrast by Nuclear Quadrupole Enhancement Relaxation, 9/2015-8/2018, Toxicity testing WP5

 

 

EUROPEAN UPCONVERSION NETWORK

COST Action CM1403 (2014-2018)

Chair: dr Hans Gorris (University of Regensburg, Germany)

Vice-Chair: prof.Tero Soukka (University of Turku, Finland)

COST Science Officer: Dr. Lucia Forzi (Bruseels, Belgium)

STSMs Manager: dr hab. Artur Bednarkiewicz (PAS & WCB EIT+, Poland)

CONTACT

info @ ucnp.eu

http://www.cost.eu/COST_Actions/cmst/CM1403

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