THE EUROPEAN UPCONVERSION NETWORK
From the Design of Photon-Upconverting Nanomaterials to Biomedical Applications : COST CM1403
PLACE OF WORK:
NanoBioPhotonics (nanofret.com), Université Paris-Sud, Orsay, France
Phone : +33 1 69 15 55 81
Email : niko.hildebrandt (at) u-psud.fr
COST ROLE : Member of the Management Committee, Leader of Work Group 2
DESCRIPTION OF THE ORGANISATIONS AND PI
Université Paris-Sud (www.u-psud.fr) is particularly recognized for the high level of its basic research, most notably in mathematics and physics. In Health Sciences, its research has had rich and fruitful results in therapeutic innovation in a large number of medical fields. Its Law, Economics and Management teams carry out highly original research in fields such as new technologies and innovation. Finally, a large number of interdisciplinary research projects are also being carried out in fields such as sport and human motricity, for example. All this research activity takes place in laboratories, which are shared for the most part with major research organizations including CNRS, Inserm and CEA, whose involvement has considerably enhanced the international reputation enjoyed by our research facilities. Université Paris-Sud is renowned not only for its research but also for the extent and variety of its courses and programmes, both in academic disciplines at Bachelor, Master’s, and PhD levels, and in technical training. Multidisciplinary curricula with teaching supported by cutting-edge research combine with innovative teaching to accommodate a wide variety of students from newcomers in their first year to those undertaking lifelong learning. Programmes focus on the needs of society and, even at doctoral level, on training students to enter the job market. The University's exceptional living and working environment provides students and young scientists with the best possible conditions for acquiring state-of-the-art knowledge and skills and enjoying student life. Key numbers: nearly 30,000 students, 1,800 teaching staff, 1,300 engineers, technicians, administrative staff, and maintenance staff,1,200 research scientists, and 900 technical and administrative staff from national research organisations (CNRS, INSERM, INRA, CEA). Annual consolidated budget of 366 million euros (132 million euros for research, 128 million euros for training and education).
The NanoBioPhotonics (nanofret.com) group at the Institut d’Electronique Fondamentale (www.ief.u-psud.fr) and the Institute of Integrative Biology of the Cell (www.i2bc.paris-saclay.fr) is a young interdisciplinary team comprised of chemists, physicists, and biochemists who are joining their expertises to create and develop novel biosensing technologies and integrate these into real-life bioapplications in cooperation with other academic groups and industrial partners. Our main focus is the application of Förster Resonance Energy Transfer (FRET) using lanthanide complexes and quantum dot nanocrystals. Using time resolved optical spectroscopy and imaging technologies we fully exploit the unique advantages of these luminescent markers for ultra-sensitive multiplexed detection, e.g., for in vitro diagnostics or live cell investigations.
Niko Hildebrandt received a Ph.D. in Physical Chemistry in 2007 under Prof. Hans-Gerd Löhmannsröben (Universität Potsdam, Germany), where he also carried out postdoctoral research until 2008. From 2008 to 2010 he was head of the group NanoPolyPhotonics at the Fraunhofer Institute for Applied Polymer Research. Since 2010 he has been Full Professor at Université Paris-Sud in Orsay, France, where he is leading the group of NanoBioPhotonics at the Institut d’Electronique Fondamentale and the Institute of Integrative Biology of the Cell with a research focus on time-resolved FRET spectroscopy and imaging for nanobioanalysis. Since 2014 he has been a member of the Institut Universitaire de France.
Description and scientific profiles of the group
Vivid and successful research is based on creativity and strong and respectful cooperation among different disciplines and collaborators. In our lab and in cooperation with our various partners from academia and the industry we follow this approach to successfully bring the initial idea to a successful result. Our research approach combines:
Optical Spectroscopy and Microscopy in the 250 – 850 nm wavelength range
Steady-state: 1 Absorbance, 1 Absorbance plate reader, 1 Luminescence, 1 plate reader
Time-resolved: 5 plate readers, 1 Luminescence
2 inverted microscopes with different objectives (10x – 60x) and cameras equiped for fixed and live cell imaging (in vitro cultured cells and tissues) and microinjection.
Steady-state cameras: 1 sCMOS, 1 EMCCD, 1CCD-B/W, 1CCD-colour
Time-resolved camera: ICCD camera (ns – ms)
Steady-state excitation: HBO lamp, NIR lasers (808 and 980nm)
Pulsed excitation: Xe-flash lamps (rep.rate < 1kHz), UV lasers (rep.rate < 300Hz), NIR lasers (808 and 980 nm), Supercontinuum source (rep. rate 100kHz - 40MHz, 400nm – 3µm), diode laser (rep. Rate single shot – 80 MHz, 405nm)
Biomolecules and Biochemistry
Well-equiped biochemical lab for conjugation of biomolecules (e.g., antibodies, streptavidin, biotin, peptides, DNA, RNA) and fluorophores incl. chemical hoods (e.g., for nanoparticle preparation), prokaryotic and eukaryotic cell culture, centrifuges, shakers, incubators, thermocycler (PCR), sterelizer (autoclave), sonicators, electroporator, separation techniques (gel electrophoresis, columns etc.), ultrapure water, solvents, buffers, serum, plasma, bioconjugation materials, …
Most significant recently published papers:
Related ongoing research grants:
Chair: dr Hans Gorris (University of Regensburg, Germany)
Vice-Chair: prof.Tero Soukka (University of Turku, Finland)
COST Science Officer: Dr. Lucia Forzi (Bruseels, Belgium)
STSMs Manager: dr hab. Artur Bednarkiewicz (PAS & WCB EIT+, Poland)
web page : Artur Bednarkiewicz Copyright © 2016 All Rights Reserved